An In Vitro Study on the Antibacterial Effects of Chlorhexidine-Loaded Positively Charged Silver Nanoparticles on Enterococcus faecalis

This study successfully developed a positively charged silver nanocomplex as nanocarrier for chlorhexidine (CHX) using ionic liquids. can interestingly deliver the antibacterial agent with synergistic effect. In this study, we synthetized and characterized (AgNPs+) CHX-loaded nanoparticles (CHX@AgNPs+) UV-visible spectroscopy, transmission electron microscopy, X-ray diffractometer, Fourier transform infrared Zetasizer. Then, loading efficiency release profile of XHX from were evaluated. The activity was evaluated by employing two standard microdilution tests to obtain minimum bactericidal inhibitory concentrations. average sizes 27.43 nm 29.66 obtained AgNPs+ CHX@AgNPs+, respectively. CHX@AgNPs+ showed constant CHX, making them more effective against Enterococcus faecalis (E. faecalis) than CHX or alone. Antibacterial assays that significantly reduced viability bacterial strain compared irrigant. had an effect similar only at intermediate concentrations (12 25 μg/mL), their effects less those other (3, 6, 50, 100 μg/mL). statistically greater all tested. MIC values 50 μg/mL. However, not value tested Therefore, designed be regarded potential root canal disinfectant clinical applications infections.